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欧盟EU FP7 项目: TESTA-VideometerLab种子健康多光谱无损检测系统

病和害虫通过种子这种途径传入到欧洲国家,采用新出现的革新性方法可改善种子品质监控。

多光谱成像系统针对TESTA项目将开发一系列方法来支持疾病和害虫控制,包括开发快速、更精确方法评估种子疾病传播,采用经济和实践性取样方法来实现大批次、低水准疾病害虫检测,使用更有效和可持续去感染方法。经过EPPO, ISHI-ISF和ISTA的咨询指导,已经鉴别了目标作物和目标疾病/害虫的组合情况。该项目成果包括建立种子传播疾病和害虫的综合性电子数据库,出据了评估去感染方法效能的验证规程以及发表了诸多科学出版物。这将为欧洲种子检测试验室和健康服务部门提供支持方法和材料。

联合团队组成包括长期在欧盟和国家政府资助的主要研究项目中任职经验研究者,来自EPPO和ISTA 植物健康小组成员、ISHI工作组、种子检验实验室和中小型种子生产企业。团队还有一名来自南非的非欧盟的国际种子生产专家,他将对出现风险进行前瞻评估。因有这么多重要参与加入到项目管理,确保了结果目标导向性好,实践性强,确保了项目的合理性。

在种子工业领域使用快速、省时的健康诊断和鉴别技术将极大加速分析、特征描述以及新品系应用。研究人员利用多光谱成像技术以及单粒近红外光谱分析法 (SKNIR) 来测量冬小麦健康以及区分冬小麦品系 (Triticum aestivum L.) 以及冬小黑麦。使用VideometerLab多光谱成像系统((405–970 nm 波长)可区分镰刀菌(Fusarium sp.)以及 黑点病感染的种子表面。另外可结合 SKNIR法来基于光谱特征不同来区分36个品系。 

1564363994101256.png

健康种子与感染种子的比较图像比较

图中种子被黑点病(Alternaria sp.) 和镰刀菌感染(Fusarium sp.) (A) RGB-图像(B) nCDA-转换图 (C) 种子培养后RGB图像,https://doi.org/10.1371/journal.pone.0152011.g001

Healthy seed are key to high crop yields, underpinning European and global food security. A wide range of diseases and pests are carried by seed and as well as spreading and increasing old problems, new problems may be introduced into the European Community countries via this route. There are currently opportunities to improve seed quality control by implementing emerging novel methodologies. The TESTA project will develop a range of novel methods to underpin the control of these diseases and pests, including faster, more accurate methods to assess the mode of seed transmission, economic and practical sampling approaches for the detection of low levels in large seed lots, novel and efficient generic detection methodologies, non-destructive testing methods and improved, effective and sustainable disinfection methods. Target crop and disease/pest combinations have been identified in consultation with EPPO, ISHI-ISF and ISTA. Outcomes from the project will include a comprehensive electronic database of seed transmitted diseases and pests, validated detection methods for target species, a validation protocol for assessing the efficacy of disinfection, as well as many key scientific publications. These will provide supporting methods and sources for the EU seed testing laboratories and plant health services.

The consortium comprises experienced researchers who have been involved in key previous research projects funded by the EU and national authorities, representatives of EPPO, ISTA plant health panel and ISHI working groups as well as seed testing laboratories and SMEs involved in seed production. The consortium includes a member from South Africa who is an international expert on seed production in non-EU countries and will provide insight into emerging risks. Involvement of these important players in the management of the project will guarantee that the project plans and outcomes are well-targeted and taken up in a practice so that the project legacy is ensured.

参考文章请详见:

The Use of Image-Spectroscopy Technology as a Diagnostic Method for Seed Health Testing and Variety Identification

Published: March 24, 2016,https://doi.org/10.1371/journal.pone.0152011

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