在种子工业领域使用快速、省时的健康诊断和鉴别技术将极大加速分析、特征描述以及新品系应用。研究人员利用多光谱成像技术以及单粒近红外光谱分析法 (SKNIR) 来测量冬小麦健康以及区分冬小麦品系 (Triticum aestivum L.) 以及冬小黑麦。使用VideometerLab多光谱成像系统（(405–970 nm 波长)可区分镰刀菌（Fusarium sp.）以及 黑点病感染的种子表面。另外可结合 SKNIR法来基于光谱特征不同来区分36个品系。
图中种子被黑点病(Alternaria sp.) 和镰刀菌感染（Fusarium sp.） (A) RGB-图像(B) nCDA-转换图 (C) 种子培养后RGB图像，https://doi.org/10.1371/journal.pone.0152011.g001
Healthy seed are key to high crop yields, underpinning European and global food security. A wide range of diseases and pests are carried by seed and as well as spreading and increasing old problems, new problems may be introduced into the European Community countries via this route. There are currently opportunities to improve seed quality control by implementing emerging novel methodologies. The TESTA project will develop a range of novel methods to underpin the control of these diseases and pests, including faster, more accurate methods to assess the mode of seed transmission, economic and practical sampling approaches for the detection of low levels in large seed lots, novel and efficient generic detection methodologies, non-destructive testing methods and improved, effective and sustainable disinfection methods. Target crop and disease/pest combinations have been identified in consultation with EPPO, ISHI-ISF and ISTA. Outcomes from the project will include a comprehensive electronic database of seed transmitted diseases and pests, validated detection methods for target species, a validation protocol for assessing the efficacy of disinfection, as well as many key scientific publications. These will provide supporting methods and sources for the EU seed testing laboratories and plant health services.
The consortium comprises experienced researchers who have been involved in key previous research projects funded by the EU and national authorities, representatives of EPPO, ISTA plant health panel and ISHI working groups as well as seed testing laboratories and SMEs involved in seed production. The consortium includes a member from South Africa who is an international expert on seed production in non-EU countries and will provide insight into emerging risks. Involvement of these important players in the management of the project will guarantee that the project plans and outcomes are well-targeted and taken up in a practice so that the project legacy is ensured.
The Use of Image-Spectroscopy Technology as a Diagnostic Method for Seed Health Testing and Variety Identification
Published: March 24, 2016，https://doi.org/10.1371/journal.pone.0152011